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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Anti-MLST8 Antibody

HPA041841
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMLST8
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MLST8 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64223
  • Target name
    MLST8
  • Target description
    MTOR associated protein MLST8
  • Target synonyms
    GBL, GbetaL, LST8, POP3, WAT1, target of rapamycin complex subunit LST8, MTOR associated protein, LST8 homolog, TORC subunit LST8, gable, mammalian lethal with SEC13 protein 8, protein GbetaL
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BVC4
  • Protein Name
    Target of rapamycin complex subunit LST8
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MLST8 using anti-MLST8 antibody (A05357-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLST8 antigen affinity purified polyclonal antibody (Catalog # A05357-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLST8 at approximately 36 kDa. The expected band size for MLST8 is at 36 kDa.
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Lane 1: Human 293T cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human K562 cell lysates; Lane 4: Human 22Rv1 cell lysates; Lane 5: Mouse brain lysates; Lane 6: Mouse kidney lysates; Lane 7: Mouse Thymus lysates; Lane 8: Rat brain lysates; Lane 9: Rat testis lysates probed with MLST8 Polyclonal Antibody, Unconjugated (bs-55132R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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