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Anti-MMACHC [N230/21]

Ab02156-1.1
Absolute Antibody
ApplicationsWestern Blot
Product group Antibodies
TargetMMACHC
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-MMACHC [N230/21]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody was used to preform a western blot on lysated mouse embryos (Moreno-Garcia et al, 2014; PMID:24889031).
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    N230/21
  • Gene ID25974
  • Target name
    MMACHC
  • Target description
    metabolism of cobalamin associated C
  • Target synonyms
    alkylcobalamin:glutathione S-alkyltransferase; cblC; cyanocobalamin reductase (cyanide-eliminating); cyanocobalamin reductase / alkylcobalamin dealkylase; methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria; methylmalonic aciduria and homocystinuria type C protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9Y4U1
  • Protein Name
    Methylmalonic aciduria and homocystinuria type C protein
  • Reactivity Supplier
    human, mouse
  • Reactivity Supplier Note
    This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 1-282 (full-length) of human MMACHC.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MMACHC using anti-MMACHC antibody (A03398-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMACHC antigen affinity purified polyclonal antibody (Catalog # A03398-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMACHC at approximately 32 kDa. The expected band size for MMACHC is at 32 kDa.
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