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Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts.

Anti-MMP15 Antibody

HPA040390
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMMP15
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MMP15
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4324
  • Target name
    MMP15
  • Target description
    matrix metallopeptidase 15
  • Target synonyms
    MMP-15, MT2-MMP, MT2MMP, MTMMP2, SMCP-2, matrix metalloproteinase-15, MT-MMP 2, matrix metallopeptidase 15 (membrane-inserted), matrix metalloproteinase 15 (membrane-inserted), membrane-type matrix metalloproteinase 2, membrane-type-2 matrix metalloproteinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51511
  • Protein Name
    Matrix metalloproteinase-15
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of MT2-MMP/MMP15 using anti-MT2-MMP/MMP15 antibody (A06396-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MT2-MMP/MMP15 antigen affinity purified polyclonal antibody (Catalog # A06396-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MT2-MMP/MMP15 at approximately 74KD. The expected band size for MT2-MMP/MMP15 is at 74KD.
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