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Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.

Anti-MOCS1 Antibody

HPA058177
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMOCS1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MOCS1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4337
  • Target name
    MOCS1
  • Target description
    molybdenum cofactor synthesis 1
  • Target synonyms
    MIG11, MOCOD, MOCS1A, MOCS1B, molybdenum cofactor biosynthesis protein 1, MOCS1A enzyme, cell migration-inducing gene 11 protein, molybdenum cofactor biosynthesis protein A, molybdenum cofactor synthesis-step 1 protein A-B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NZB8
  • Protein Name
    Molybdenum cofactor biosynthesis protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MOCS1 using anti-MOCS1 antibody (A07628-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human 293T whole cell lysates, Lane 7: human SH-SY5Y whole cell lysates, Lane 8: human RT4 whole cell lysates, Lane 9: rat liver tissue lysates, Lane 10: rat PC-12 whole cell lysates, Lane 11: mouse liver tissue lysates, Lane 12: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOCS1 antigen affinity purified polyclonal antibody (Catalog # A07628-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MOCS1 at approximately 58 kDa. The expected band size for MOCS1 is at 70 kDa.
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