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Anti-mouse apoA1 mAb (mHDL36), biotin

Anti-mouse apoA1 mAb (mHDL36), biotin

3750-6
Mabtech
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse
TargetApoa1
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Overview

  • Supplier
    Mabtech
  • Product Name
    Anti-mouse apoA1 mAb (mHDL36), biotin
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    mHDL36
  • Conjugate
    Biotin
  • Gene ID11806
  • Target name
    Apoa1
  • Target description
    apolipoprotein A-I
  • Target synonyms
    Alp-1, Apoa-1, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2, Sep2, apo-AI, apoA-I, apolipoprotein A-I, apolipoprotein A1
  • Host
    Rat
  • Isotype
    IgG2a
  • Scientific Description
    Validated for our assays. - This monoclonal antibody enables specific detection of mouse apoA1 in immunoassays such as ELISA and Western blot.
  • Reactivity
    Mouse
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of APOA1 using anti-APOA1 antibody (PB9844). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOA1 antigen affinity purified polyclonal antibody (Catalog # PB9844) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOA1 at approximately 24 kDa. The expected band size for APOA1 is at 31 kDa.
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