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Anti-Mouse AQP8 Antibody

144-08539
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetAQP8
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Mouse AQP8 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID343
  • Target name
    AQP8
  • Target description
    aquaporin 8
  • Target synonyms
    AQP-8, aquaporin-8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO94778
  • Protein Name
    Aquaporin-8
  • Scientific Description
    AQP8 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AQP8 using anti-AQP8 antibody (PA1511). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AQP8 antigen affinity purified polyclonal antibody (Catalog # PA1511) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AQP8 at approximately 27KD. The expected band size for AQP8 is at 27KD.
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The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA186529(AQP8 Antibody) at dilution 1/75, on the right is treated with synthetic peptide. (Original magnification: x200)
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