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Anti- mouse Bim [ham151-149]

AB03542-1.1-BT
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
Product group Antibodies
TargetBcl2l11
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti- mouse Bim [ham151-149]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody reacted in Western blots and by immunofluorescence with permeabilized B and T cells from Bim +/+ mice (Oliver et al., 2004; PMID: 15520248). T cells were analyzed for levels of Bcl-2 and Bim protein by staining of permeabilized cells with the antibody (Jorgensen et al., 2007; PMID: 17785775). The antibody was used for flow cytometry analysis (Kashiwakura et al., 2013; PMID: 23278598).
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ham151-149
  • Gene ID12125
  • Target name
    Bcl2l11
  • Target description
    BCL2 like 11
  • Target synonyms
    1500006F24Rik, Bim, Bod, bcl2-L-11, bcl-2-like protein 11, BCL2-like 11 (apoptosis facilitator), Bcl2 interacting mediator of cell death, bcl2-interacting mediator of cell death
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO54918
  • Protein Name
    Bcl-2-like protein 11
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Hamster IgG format for improved compatibility with existing reagents assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Bim/Bcl2l11 using anti-Bim/Bcl2l11 antibody (A01552-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bim/Bcl2l11 antigen affinity purified polyclonal antibody (Catalog # A01552-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bim/Bcl2l11 at approximately 22 kDa. The expected band size for Bim/Bcl2l11 is at 22 kDa.
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