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Anti-Mouse CD86 Antibody, Purified

135-00041
RayBiotech
ApplicationsFlow Cytometry
Product group Antibodies
TargetCd86
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Mouse CD86 Antibody, Purified
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GL1
  • Gene ID12524
  • Target name
    Cd86
  • Target description
    CD86 antigen
  • Target synonyms
    activation B7-2 antigen; B7; B7.2; B70; B7-2; Cd28l; Cd28l2; CLS1; early T cell costimulatory molecule-1; ETC-1; Ly5; Ly-5; Ly58; Ly-58; MB7; MB7-2; T-lymphocyte activation antigen CD86; TS/A-2
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDP42082
  • Protein Name
    T-lymphocyte activation antigen CD86
  • Scientific Description
    Mouse CD86 Antibody [clone: GL1], monoclonal Rat IgG2a kappa. Validated for flow cytometry.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cd86 using anti-Cd86 antibody (A00220-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd86 antigen affinity purified polyclonal antibody (Catalog # A00220-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd86 at approximately 60-80 kDa. The expected band size for Cd86 is at 35 kDa.
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