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Western blot analysis of lysates from mouse kidney, mouse lung, mouse testis tissue lysate (from left to right), using Epcam Antibody (C-term). A00276 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Western blot analysis of lysates from mouse kidney, mouse lung, mouse testis tissue lysate (from left to right), using Epcam Antibody (C-term). A00276 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Western blot analysis of lysates from mouse kidney, mouse lung, mouse testis tissue lysate (from left to right), using Epcam Antibody (C-term). A00276 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

Anti-(Mouse) Epcam Antibody (C-term)

A00276
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityMouse, Rat
TargetEpcam
400 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-(Mouse) Epcam Antibody (C-term)
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID17075
  • Target name
    Epcam
  • Target description
    epithelial cell adhesion molecule
  • Target synonyms
    CD326, EGP, EGP-2, Egp314, Ep-CAM, EpCAM1, GA733-2, Ly74, TROP1, Tacsd1, Tacstd1, gp40, epithelial cell adhesion molecule, Trop-1 protein, epithelial glycoprotein 314, lymphocyte antigen 74, mEGP314, panepithelial glycoprotein 314, protein 289A, tumor-associated calcium signal transducer 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99JW5
  • Protein Name
    Epithelial cell adhesion molecule
  • Scientific Description
    Boster Bio Anti-(Mouse) Epcam Antibody (C-term) (Catalog # A00276). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Mouse.
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. IHC analysis of EpCAM using anti-EpCAM antibody (A00276-1). EpCAM was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2microg/ml rabbit anti-EpCAM Antibody (A00276-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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