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Anti-mouse IL-22 mAb (MT231), biotin

Anti-mouse IL-22 mAb (MT231), biotin

3376-6
Mabtech
ApplicationsELISpot Assay
Product group Antibodies
TargetIl22
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Overview

  • Supplier
    Mabtech
  • Product Name
    Anti-mouse IL-22 mAb (MT231), biotin
  • Delivery Days Customer
    5
  • Applications
    ELISpot Assay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MT231
  • Conjugate
    Biotin
  • Gene ID50929
  • Target name
    Il22
  • Target description
    interleukin 22
  • Target synonyms
    IL-22, IL-22a, ILTIFa, If2b1, Iltif, interleukin-22, IL-10-related T-cell-derived-inducible factor, IL-TIF alpha, ILTIF alpha, interferon 2b1, interleukin 10-related T cell-derived inducible factor, interleukin-22a
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Validated for our assays. - This monoclonal antibody enables specific detection of mouse IL-22 in ELISpot and FluoroSpot.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL22 using anti-IL22 antibody (A00963-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse kidney tissue lysate, Lane 2: mouse brain tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL22 antigen affinity purified polyclonal antibody (Catalog # A00963-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL22 at approximately 20KD. The expected band size for IL22 is at 20KD.
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