Anti-Mouse Nidogen/enactin [JF2 (1E2-A5-A3)]

Absolute Antibody

Anti-Mouse Nidogen/enactin [JF2 (1E2-A5-A3)]

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This antibody is specific for the G3 domain of mouse nidogen-1. Nidogen-1 is a sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extra

To characterize the original mouse version of this antibody ELISA was preformed against recombinant mouse nidogen-1 (Ries et al, 2001; pmid:11589703). Futhermore binding assays on mouse nidogen-1 were also preformed using the mouse version of this antibody (Ries et al, 2001; pmid:11589703). And finally this antibody was tested using surface plasmon resonance with mouse nidogen-1 as antigen (Ries et al, 2001; pmid:11589703). A tube-like formation assay was preformed on HMEC-1 cells. To study the influence of nidogen-1 in the process the mouse version of this antibody was used (Titz et al, 2004; pmid:15241558).

Functional Assay, ELISA, Other Application

ELISA; SPR; functional assays

Research Use Only

Monoclonal

JF2 (1E2-A5-A3)

Nid1

nidogen 1

A630025O17; enta; entact; entactin; entactin 1; entactin-1; Nid; NID-1; nidog; Nidogen precursor (Entactin); nidogen-1

Rabbit

IgG

Nidogen-1

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Mouse

Mouse

The antibody was raised by immunizing fisher rats with recombinant mouse nidogen-1.

-20°C,2°C to 8°C

12352203