Anti-Mouse Nidogen/enactin [JF3 (4H5-B2-E4)]

Absolute Antibody

Anti-Mouse Nidogen/enactin [JF3 (4H5-B2-E4)]

7

This antribody is specific for the G2 domain of nidogen-1. It binds in such a way that it is able to stop the binding of nidogen to laminin. Nidogen-1 is a sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin.

The binding of the mouse version of this antibody to nidogen-1 was proven by ELISA. Further, high binding affinity of the mouse version of this antibody to nidogen-1 was demonstrated using surface plasmon resonance (Ries et al, 2001; pmid:11589703). Frozen sections of fetal, newborn, and 4-week-old mice were stained using the mouse version of this antibody to use for immunohistochemistry. This was performed while researching Collagen XXVIII (Veit et al, 2006; pmid:16330543). Frozen and paraffin-embedded sections of fetal, newborn, 3-week-old, and adult mice were stained using the mouse version of this antibody to use for immunohistochemistry. This assay was preformed while researching a protein called AMACO (Sengle et al, 2003; pmid:14506275). The mouse version of this antibody was used to stain whole mouse embryos or dissected organs from mice for immunohistochemistry. The staining allowed for the immunolocalization of nidogen-1 (Kostka et al, 2001; pmid:11564885).

ELISA, ImmunoHistoChemistry, Other Application

ELISA; SPR; IHC

Research Use Only

Monoclonal

JF3 (4H5-B2-E4)

Nid1

nidogen 1

A630025O17; enta; entact; entactin; entactin 1; entactin-1; Nid; NID-1; nidog; Nidogen precursor (Entactin); nidogen-1

Rat

IgG2a

Nidogen-1

Human, Mouse

Human, Mouse

This antibody was raised by immunizing a rat with G1/link region of mouse nidogen that is able to block its binding to laminin.

-20°C,2°C to 8°C

12352203