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Anti-Mouse SIGLEC12 Antibody

144-08519
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetSIGLEC12
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Mouse SIGLEC12 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID89858
  • Target name
    SIGLEC12
  • Target description
    sialic acid binding Ig like lectin 12
  • Target synonyms
    S2V; sialic acid-binding Ig-like lectin 12; sialic acid-binding Ig-like lectin-like 1; SIGLECL1; SIGLEC-like 1; Siglec-XII; SLG
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96PQ1
  • Protein Name
    Sialic acid-binding Ig-like lectin 12
  • Scientific Description
    SIGLEC12 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SIGLEC12 using anti-SIGLEC12 antibody (A10550-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat spleen tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIGLEC12 antigen affinity purified polyclonal antibody (Catalog # A10550-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIGLEC12 at approximately 70 kDa. The expected band size for SIGLEC12 is at 65 kDa.
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The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using CSB-PA812859(SIGLEC12 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: x200)
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