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Anti-Mouse/Rat MC3R Antibody

144-11609
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetMC3R
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Mouse/Rat MC3R Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4159
  • Target name
    MC3R
  • Target description
    melanocortin 3 receptor
  • Target synonyms
    BMIQ9, MC3, MC3-R, OB20, OQTL, melanocortin receptor 3, obesity quantitative trait locus
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41968
  • Protein Name
    Melanocortin receptor 3
  • Scientific Description
    MC3R Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MC3 Receptor using anti-MC3 Receptor antibody (A02841-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: mouse kidney tissue lysate, lane 2: COLO320 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MC3 Receptor antigen affinity purified polyclonal antibody (Catalog # A02841-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MC3 Receptor at approximately 40KD. The expected band size for MC3 Receptor is at 36KD.
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IHC image of CSB-PA013560LA01HU diluted at 1:200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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