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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Anti-MPPE1 Antibody

HPA012639
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMPPE1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MPPE1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID65258
  • Target name
    MPPE1
  • Target description
    metallophosphoesterase 1
  • Target synonyms
    Cdc1, PGAP5, metallophosphoesterase 1, metallo phosphoesterase, post-GPI attachment to proteins 5, post-GPI attachment to proteins factor 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ53F39
  • Protein Name
    Metallophosphoesterase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MPPE1 using anti-MPPE1 antibody (A12491-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPPE1 antigen affinity purified polyclonal antibody (Catalog # A12491-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPPE1 at approximately 45 kDa. The expected band size for MPPE1 is at 45 kDa.
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