Anti-MPXV A35 protein [MPXV-mAb 975], Human IgM, kappa

Absolute Antibody

Anti-MPXV A35 protein [MPXV-mAb 975], Human IgM, kappa

9

This antibody binds the C-terminal domain of A35 protein (163-TSDYQDSDVSQEVRKYFCT-181) of the monkeypox virus and also cross reacts with A33 protein of vaccinia virus. Mpox is an infectious disease caused by the monkeypox virus which can be transmitted to

The original human IgG antibody binds monkeypox virus A35 protein with a very high binding affinity of less than 1 pM as measured by SPR. This antibody can bind MPXV-A35 and VACV-A33 in an ELISA. The epitope characterization of this antibody was performed using flow cytometry by constructing several C-terminal truncated A35 variants and expressed them individually on the surface of 293T cells by transient transfection. This antibody can recognize monkeypox virus A35 protein and vaccinia virus A33 protein in a western blot assay. This antibody can be used to recognize live vaccinia virus (VACV) in an immunofluorescence assay. The cellular localizations of A33 in the VACV-infected Vero E6 cells was clearly visualized using this antibody (PMID: 37230974).

Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA

Research Use Only

Monoclonal

MPXV-mAb 975

Human

IgM

Virus

-20°C,2°C to 8°C

12352203