Anti-MS4A4A [5C12]

Absolute Antibody

Anti-MS4A4A [5C12]

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This antibody is specific for human extracellular MS4A4A. 5C12 mAb only partially cross-blocked either 4H2 (Ab01373) and 3F2 (Ab01375) and therefore probably detects a unique or overlapping epitope (Sanyal et al., 2017; pmid: 28303902). MS4A (membrane-spa

5C12 is recommended for the detection and analysis of expression of MS4A4A. Importantly, MS4A4A has been shown to be a marker for M2 macrophages and plasma cells and to be overexpressed in multiple myelomas, suggesting a potential role in targeting alternatively activated macrophages such as tumor-associated macrophages, and in the treatment of multiple myeloma and mantle cell lymphoma (Sanyal et al., 2017; pmid: 28303902). The antibody 5C12 can be successfully used for the detection of MS4A4A-expressing cells via flow cytometry (Sanyal et al., 2017; pmid: 28303902). Finally, specificity of this antibody was additionally confirmed via immunoprecipitation of lysates of BJAB MS4A4A-HA cells (Sanyal et al., 2017; pmid: 28303902).

Flow Cytometry, ImmunoPrecipitation

FC; IP

Research Use Only

Monoclonal

5C12

MS4A4A

membrane spanning 4-domains A4A

4SPAN1; CD20 antigen-like 1; CD20L1; CD20-L1; epididymis secretory sperm binding protein; Fc epsilon receptor beta subunit homolog; four-span transmembrane protein 1; HDCME31P; membrane-spanning 4-domains subfamily A member 4A; membrane-spanning 4-domains, subfamily A, member 4; MS4A4; MS4A7

Rabbit

IgG

Membrane-spanning 4-domains subfamily A member 4A

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

This antibody was generated by immunizing C57BL/6 mice against mouse cell line 300-19 expressing transfected human membrane-spanning 4-domains subfamily A member 4A (MS4A4A)-GFP.

-20°C,2°C to 8°C

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