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Anti-MSH2

Y050845
Applied Biological Materials
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
100 ug
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Overview

  • Supplier
    Applied Biological Materials
  • Product Name
    Anti-MSH2
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 ug/ul
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    MSH2 is a 100 kDa nuclear antigen and encodes a protein of 934 amino acids. The MSH2 gene is one of 4 known genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma. MSHS mutations are responsible for 50% of inherited non-polyposis colorectal (HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Lane 1: Mouse Thymus tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Mouse NIH/3T3 cell lysates; Lane 4: Human Hela cell lysates; Lane 5: Human SW480 cell lysates probed with MSH2 Polyclonal Antibody, Unconjugated (bs-0758R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of MSH2 using anti-MSH2 antibody (A00140-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSH2 antigen affinity purified polyclonal antibody (Catalog # A00140-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSH2 at approximately 105 kDa. The expected band size for MSH2 is at 105 kDa.
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