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Immunohistochemical staining of human cerebellum shows moderate nuclear positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows moderate nuclear positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows moderate nuclear positivity in Purkinje cells.

Anti-MSL3 Antibody

HPA034536
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMSL3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MSL3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10943
  • Target name
    MSL3
  • Target description
    MSL complex subunit 3
  • Target synonyms
    MRSXBA, MRXS36, MRXSBA, MSL3L1, MSL complex subunit 3, MSL3-like 1, male-specific lethal 3 homolog, male-specific lethal-3 protein-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N5Y2
  • Protein Name
    MSL complex subunit 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MSL3 using anti-MSL3 antibody (A04993-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human CCRF-CEM whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSL3 antigen affinity purified polyclonal antibody (Catalog # A04993-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSL3 at approximately 70 kDa. The expected band size for MSL3 is at 60 kDa.
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