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Anti-MTA2 [RAB-T15]

Ab01813-1.1
Absolute Antibody
ApplicationsImmunoFluorescence, ImmunoPrecipitation
Product group Antibodies
ReactivityHuman
TargetMTA2
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-MTA2 [RAB-T15]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody has been used and recommended for the following methods: IP-MS; IF.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation
  • Applications Supplier
    IP-MS; IF
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RAB-T15
  • Gene ID9219
  • Target name
    MTA2
  • Target description
    metastasis associated 1 family member 2
  • Target synonyms
    MTA1L1, PID, metastasis-associated protein MTA2, MTA1-L1 protein, metastasis -associated gene 1-like 1, metastasis associated gene family, member 2, metastasis-associated 1-like 1, metastasis-associated protein 2, p53 target protein in deacetylase complex
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO94776
  • Protein Name
    Metastasis-associated protein MTA2
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was obtained by recombinant antibody (rAb) phage display recognizing MTA2 protein under non-denaturing conditions.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MTA2 using anti-MTA2 antibody (A03073-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTA2 antigen affinity purified polyclonal antibody (Catalog # A03073-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTA2 at approximately 75KD. The expected band size for MTA2 is at 75KD.
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