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Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.

Anti-MTCH2 Antibody

HPA038390
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetMTCH2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MTCH2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23788
  • Target name
    MTCH2
  • Target description
    mitochondrial carrier 2
  • Target synonyms
    2310034D24Rik; HSPC032; met-induced mitochondrial protein; MIMP; mitochondrial carrier homolog 2; SLC25A50; solute carrier family 25, member 50
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y6C9
  • Protein Name
    Mitochondrial carrier homolog 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA083456(MTCH2 Antibody) at dilution 1/45, on the right is treated with fusion protein. (Original magnification: x200)
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Figure 1. Western blot analysis of MTCH2 using anti-MTCH2 antibody (A05288-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTCH2 antigen affinity purified polyclonal antibody (Catalog # A05288-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTCH2 at approximately 33 kDa. The expected band size for MTCH2 is at 33 kDa.
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