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Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic/nuclear positivity in urothelial cells with additional membranous positivity.
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic/nuclear positivity in urothelial cells with additional membranous positivity.
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic/nuclear positivity in urothelial cells with additional membranous positivity.

Anti-MTCL1 Antibody

HPA046245
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMTCL1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MTCL1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23255
  • Target name
    MTCL1
  • Target description
    microtubule crosslinking factor 1
  • Target synonyms
    CCDC165, KIAA0802, SOGA2, microtubule cross-linking factor 1, PAR-1-interacting protein, SOGA family member 2, coiled-coil domain containing 165, coiled-coil domain-containing protein 165
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y4B5
  • Protein Name
    Microtubule cross-linking factor 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MTCL1 using anti-MTCL1 antibody (A10954-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat ovary tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTCL1 antigen affinity purified polyclonal antibody (Catalog # A10954-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTCL1 at approximately 270-300 kDa. The expected band size for MTCL1 is at 210 kDa.
Product group Antibodies
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ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMTCL1
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