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Immunohistochemical staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.

Anti-MTHFD1 Antibody

HPA000704
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetMTHFD1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MTHFD1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4522
  • Target name
    MTHFD1
  • Target description
    methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1
  • Target synonyms
    5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase; C-1-tetrahydrofolate synthase, cytoplasmic; C1-THF synthase; CIMAH; cytoplasmic C-1-tetrahydrofolate synthase; epididymis secretory sperm binding protein; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase; MTHFC; MTHFD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11586
  • Protein Name
    C-1-tetrahydrofolate synthase, cytoplasmic
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MTHFD1 using anti-MTHFD1 antibody (A02025-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTHFD1 antigen affinity purified polyclonal antibody (Catalog # A02025-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTHFD1 at approximately 102 kDa. The expected band size for MTHFD1 is at 102 kDa.
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