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Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.

Anti-MTMR2 Antibody

HPA049831
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetMTMR2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MTMR2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8898
  • Target name
    MTMR2
  • Target description
    myotubularin related protein 2
  • Target synonyms
    CMT4B; CMT4B1; myotubularin-related protein 2; phosphatidylinositol-3,5-bisphosphate 3-phosphatase; phosphatidylinositol-3-phosphatase; phosphatidylinositol-3-phosphate phosphatase; phosphoinositide-3-phosphatase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13614
  • Protein Name
    Myotubularin-related protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate All lanes: MTMR2 antibody at 5.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 74, 66 kDa Observed band size: 74 kDa
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Figure 1. Western blot analysis of MTMR2 using anti-MTMR2 antibody (A04202-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTMR2 antigen affinity purified polyclonal antibody (Catalog # A04202-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTMR2 at approximately 73 kDa. The expected band size for MTMR2 is at 73 kDa.
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