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Immunohistochemical staining of human fallopian tube shows weak nucleolar positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows weak nucleolar positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows weak nucleolar positivity in glandular cells.

Anti-MUS81 Antibody

HPA059530
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetMUS81
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MUS81
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80198
  • Target name
    MUS81
  • Target description
    MUS81 structure-specific endonuclease subunit
  • Target synonyms
    crossover junction endonuclease MUS81; MUS81 endonuclease homolog; SLX3; SLX3 structure-specific endonuclease subunit homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96NY9
  • Protein Name
    Crossover junction endonuclease MUS81
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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IHC image of CSB-PA836272LA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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WB analysis of various sample lysates using GTX03656 MUS81 antibody. Lane 1 : HeLa whole cell lysates Lane 2 : A431 whole cell lysates Lane 3 : HepG2 whole cell lysates Lane 4 : rat brain tissue lysates Lane 5 : PC-12 whole cell lysates Lane 6 : brain tissue lysates Lane 7 : NIH/3T3 whole cell lysates
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Figure 1. Western blot analysis of MUS81 using anti-MUS81 antibody (A00810-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUS81 antigen affinity purified polyclonal antibody (Catalog # A00810-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUS81 at approximately 70 kDa. The expected band size for MUS81 is at 61 kDa.
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