Figure 1. Western blot analysis of MSI1 using anti-MSI1 antibody (A05052-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human COLO-320 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSI1 antigen affinity purified polyclonal antibody (Catalog # A05052-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSI1 at approximately 39KD. The expected band size for MSI1 is at 39KD.
Figure 1. Western blot analysis of MSI1 using anti-MSI1 antibody (A05052-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human COLO-320 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSI1 antigen affinity purified polyclonal antibody (Catalog # A05052-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSI1 at approximately 39KD. The expected band size for MSI1 is at 39KD.
Anti-Musashi 1/Msi1 Antibody Picoband(r)
A05052-2-CY3
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetMSI1
Overview
- SupplierBoster Bio
- Product NameAnti-Musashi 1/Msi1 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsFlow Cytometry, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateCy3
- Gene ID4440
- Target nameMSI1
- Target descriptionmusashi RNA binding protein 1
- Target synonymsmusashi1; musashi-1; RNA-binding protein Musashi homolog 1
- HostRabbit
- IsotypeIgG
- Protein IDO43347
- Protein NameRNA-binding protein Musashi homolog 1
- Scientific DescriptionBoster Bio Anti-Musashi 1/Msi1 Antibody Picoband® catalog # A05052-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203