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Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.

Anti-MVK Antibody

HPA016961
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMVK
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MVK
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4598
  • Target name
    MVK
  • Target description
    mevalonate kinase
  • Target synonyms
    LRBP, MK, MVLK, POROK3, mevalonate kinase, LH receptor mRNA-binding protein, mevalonate kinase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ03426
  • Protein Name
    Mevalonate kinase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MVK using anti-MVK antibody (A02465-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MVK antigen affinity purified polyclonal antibody (Catalog # A02465-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MVK at approximately 42 kDa. The expected band size for MVK is at 42 kDa.
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