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Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.

Anti-MYCBP Antibody

HPA041188
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMYCBP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MYCBP
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26292
  • Target name
    MYCBP
  • Target description
    MYC binding protein
  • Target synonyms
    AMY-1, C-Myc-binding protein, associate of myc-1, c-myc binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99417
  • Protein Name
    c-Myc-binding protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MYCBP using anti-MYCBP antibody (A06216-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human 293T whole cell lysates; Lane 3: rat lung tissue lysates; Lane 4: rat NRK whole cell lysates; Lane 5: mouse lung tissue lysates; Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYCBP antigen affinity purified polyclonal antibody (Catalog # A06216-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYCBP at approximately 12 kDa. The expected band size for MYCBP is at 12 kDa.
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