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Immunohistochemical staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human rectum shows moderate cytoplasmic positivity in glandular cells.

Anti-MYL5 Antibody

HPA037381
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMYL5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MYL5 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4636
  • Target name
    MYL5
  • Target description
    myosin light chain 5
  • Target synonyms
    MYLC2, myosin light chain 5, myosin regulatory light chain 5, myosin, light chain 5, regulatory, myosin, light polypeptide 5, regulatory, superfast myosin regulatory light chain 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02045
  • Protein Name
    Myosin light chain 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MYL5 using anti-MYL5 antibody (A15974). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYL5 antigen affinity purified polyclonal antibody (Catalog # A15974) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYL5 at approximately 18 kDa. The expected band size for MYL5 is at 18 kDa.
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