Anti-N type Glycophorin A [NNA7]

Absolute Antibody

Anti-N type Glycophorin A [NNA7]

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This antibody recognizes the human N-type blood-group antigen (MNS blood group) comprised of the N-terminal glycopeptide of glycophorin A (GPA). Glycophorin A is the major sialoglycoproteins and intrinsic membrane protein of the human erythrocyte which be

This antibody is a synthetic clone derives from the parental clone N72 Fab. This antibody is capable of reacting with N-type glycophorin A and NN red cells in an ELISA and an agglutination assay (PMID: 10220261). This antibody is capable of detecting glycophorin A of N-type and glycophorin B in a western blot assay (PMID: 19833240; 9574545). This antibody was also used in the immunofluorescence staining of transfected CHO cells expressing recombinant N-type GPA (PMID: 14962308).

ImmunoFluorescence, Western Blot, ELISA, Other Application

ELISA; Agglutination reaction; WB; IF

Research Use Only

Monoclonal

NNA7

GYPA

glycophorin A (MNS blood group)

CD235a; erythroid-lineage-specific membrane sialoglycoprotein; glycophorin A (MN blood group); glycophorin A, GPA; glycophorin Erik; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Sta type C; glycophorin-A; GPA; GPErik; GPSAT; HGpMiV; HGpMiXI; HGpSta(C); Mi.V glycoprotein (24 AA); MN; MN sialoglycoprotein; MNS; PAS-2; recombinant glycophorin A-B Miltenberger-DR; sialoglycoprotein alpha

Rabbit

IgG

Glycophorin-A

This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.

Human

Human

The original antibody was isolated from light-chain cDNA libraries constructed from mice immunized with N-type glycophorin A. These were inserted into a recombinant pComb3H vector containing the N92 Fd fragment. The N92 Fd fragment: light-chain libraries were panned on N-type glycophorin A or NN red cells.

-20°C,2°C to 8°C

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