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Immunohistochemical staining of human appendix shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human appendix shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human appendix shows moderate cytoplasmic positivity in glandular cells.

Anti-NAA16 Antibody

HPA040157
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNAA16
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NAA16 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79612
  • Target name
    NAA16
  • Target description
    N-alpha-acetyltransferase 16, NatA auxiliary subunit
  • Target synonyms
    NARG1L, N-alpha-acetyltransferase 16, NatA auxiliary subunit, NARG1-like protein, NMDA receptor-regulated 1-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6N069
  • Protein Name
    N-alpha-acetyltransferase 16, NatA auxiliary subunit
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NARG1L/NAA16 using anti-NARG1L/NAA16 antibody (A13221-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NARG1L/NAA16 antigen affinity purified polyclonal antibody (Catalog # A13221-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NARG1L/NAA16 at approximately 130 kDa. The expected band size for NARG1L/NAA16 is at 100 kDa.
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