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Immunohistochemical staining of human lung shows strong membranous positivity in pneumocytes.
Immunohistochemical staining of human lung shows strong membranous positivity in pneumocytes.
Immunohistochemical staining of human lung shows strong membranous positivity in pneumocytes.

Anti-NAB1 Antibody

HPA002738
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNAB1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NAB1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4664
  • Target name
    NAB1
  • Target description
    NGFI-A binding protein 1
  • Target synonyms
    EGR1 binding protein 1; EGR-1-binding protein 1; NGFI-A-binding protein 1; transcriptional regulatory protein p54
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13506
  • Protein Name
    NGFI-A-binding protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NAB1 using anti-NAB1 antibody (A06669-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAB1 antigen affinity purified polyclonal antibody (Catalog # A06669-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAB1 at approximately 56 kDa. The expected band size for NAB1 is at 54 kDa.
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