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Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Anti-NADK2 Antibody

HPA061492
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNADK2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NADK2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID133686
  • Target name
    NADK2
  • Target description
    NAD kinase 2, mitochondrial
  • Target synonyms
    C5orf33, DECRD, MNADK, NADKD1, NAD kinase 2, mitochondrial, NAD kinase domain-containing protein 1, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ4G0N4
  • Protein Name
    NAD kinase 2, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of C5orf33/NADK2 using anti-C5orf33/NADK2 antibody (A09619-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C5orf33/NADK2 antigen affinity purified polyclonal antibody (Catalog # A09619-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C5orf33/NADK2 at approximately 53 kDa. The expected band size for C5orf33/NADK2 is at 49 kDa.
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Western Blot Positive WB detected in: 293 whole cell lysate, HL60 whole cell lysate, Rat liver tissue, Mouse kidney tissue, Mouse liver tissue All lanes: NADK2 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 46, 32 kDa Observed band size: 50, 46 kDa
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