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Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts.

Anti-NAF1 Antibody

HPA036241
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNAF1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NAF1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID92345
  • Target name
    NAF1
  • Target description
    nuclear assembly factor 1 ribonucleoprotein
  • Target synonyms
    PFBMFT7, H/ACA ribonucleoprotein complex non-core subunit NAF1, nuclear assembly factor 1 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96HR8
  • Protein Name
    H/ACA ribonucleoprotein complex non-core subunit NAF1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of NAF1 using anti-NAF1 antibody (A02629-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAF1 antigen affinity purified polyclonal antibody (Catalog # A02629-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAF1 at approximately 70 kDa. The expected band size for NAF1 is at 54 kDa.
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