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Immunofluorescence staining of mouse thalamus shows neuronal immunoreactivity in paraventricular thalamic nucleus.
Immunofluorescence staining of mouse thalamus shows neuronal immunoreactivity in paraventricular thalamic nucleus.
Immunofluorescence staining of mouse thalamus shows neuronal immunoreactivity in paraventricular thalamic nucleus.

Anti-NAGLU Antibody

HPA038815
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNAGLU
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NAGLU Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4669
  • Target name
    NAGLU
  • Target description
    N-acetyl-alpha-glucosaminidase
  • Target synonyms
    CMT2V, MPS-IIIB, MPS3B, NAG, UFHSD, alpha-N-acetylglucosaminidase, N-acetylglucosaminidase, alpha, testicular tissue protein Li 18
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54802
  • Protein Name
    Alpha-N-acetylglucosaminidase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of NAGLU using anti-NAGLU antibody (A03549). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAGLU antigen affinity purified polyclonal antibody (A03549) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAGLU at approximately 82 kDa. The expected band size for NAGLU is at 82 kDa.
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