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Immunofluorescent staining of human cell line SuSa shows localization to nucleoplasm.
Immunofluorescent staining of human cell line SuSa shows localization to nucleoplasm.
Immunofluorescent staining of human cell line SuSa shows localization to nucleoplasm.

Anti-NANOG Antibody

HPA072117
Atlas Antibodies
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetNANOG
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NANOG Antibody
  • Delivery Days Customer
    4
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79923
  • Target name
    NANOG
  • Target description
    Nanog homeobox
  • Target synonyms
    homeobox protein NANOG, homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H9S0
  • Protein Name
    Homeobox protein NANOG
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of Nanog using anti-Nanog antibody (A00153-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat ovary tissue lysates, Lane 2: mouse ovary tissue lysates, Lane 3: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nanog antigen affinity purified polyclonal antibody (Catalog # A00153-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nanog at approximately 48KD. The expected band size for Nanog is at 34KD.
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