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Immunohistochemical staining of human duodenum shows strong membranous and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong membranous and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong membranous and cytoplasmic positivity in glandular cells.

Anti-NAV3 Antibody

HPA032111
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNAV3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NAV3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID89795
  • Target name
    NAV3
  • Target description
    neuron navigator 3
  • Target synonyms
    NEDSFB, POMFIL1, STEERIN3, unc53H3, neuron navigator 3, pore membrane and/or filament interacting like protein 1, unc-53 homolog 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IVL0
  • Protein Name
    Neuron navigator 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NAV3 using anti-NAV3 antibody (A07567-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human HUVEC whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAV3 antigen affinity purified polyclonal antibody (A07567-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAV3 at approximately 256 kDa. The expected band size for NAV3 is at 256 kDa.
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