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Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.

Anti-NCBP2 Antibody

HPA062483
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNCBP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NCBP2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22916
  • Target name
    NCBP2
  • Target description
    nuclear cap binding protein subunit 2
  • Target synonyms
    20 kDa nuclear cap-binding protein; CBC2; CBP20; cell proliferation-inducing gene 55 protein; NCBP 20 kDa subunit; NCBP interacting protein 1; NIP1; nuclear cap binding protein subunit 2, 20kD; nuclear cap binding protein subunit 2, 20kDa; nuclear cap-binding protein subunit 2; PIG55
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP52298
  • Protein Name
    Nuclear cap-binding protein subunit 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CBP20/NCBP2 using anti-CBP20/NCBP2 antibody (A08031-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CCRF-CEM whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBP20/NCBP2 antigen affinity purified polyclonal antibody (Catalog # A08031-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBP20/NCBP2 at approximately 18 kDa. The expected band size for CBP20/NCBP2 is at 18 kDa.
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