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Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Anti-NCOA5 Antibody

HPA050231
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNCOA5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NCOA5
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57727
  • Target name
    NCOA5
  • Target description
    nuclear receptor coactivator 5
  • Target synonyms
    CIA, bA465L10.6, nuclear receptor coactivator 5, NCoA-5, coactivator independent of AF-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HCD5
  • Protein Name
    Nuclear receptor coactivator 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, MCF-7 whole cell lysate, Mouse heart tissue, Mouse liver tissue All lanes: NCOA5 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 66 kDa Observed band size: 66, 35, 25 kDa
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Figure 1. Western blot analysis of NCOA5 using anti-NCOA5 antibody (A07810-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCOA5 antigen affinity purified polyclonal antibody (Catalog # A07810-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCOA5 at approximately 66 kDa. The expected band size for NCOA5 is at 66 kDa.
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