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Immunohistochemical staining of human cerebral cortex shows moderate nucleolar positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows moderate nucleolar positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows moderate nucleolar positivity in neuronal cells.

Anti-NDE1 Antibody

HPA024075
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNDE1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NDE1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54820
  • Target name
    NDE1
  • Target description
    nudE neurodevelopment protein 1
  • Target synonyms
    HOM-TES-87, LIS4, MHAC, NDE, NUDE, NUDE1, nuclear distribution protein nudE homolog 1, LIS1-interacting protein NUDE1, rat homolog, epididymis secretory sperm binding protein, nudE nuclear distribution E homolog 1, nudE nuclear distribution gene E homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NXR1
  • Protein Name
    Nuclear distribution protein nudE homolog 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of NDE1 using anti-NDE1 antibody (A03656-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human U937 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDE1 antigen affinity purified polyclonal antibody (Catalog # A03656-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDE1 at approximately 38 kDa. The expected band size for NDE1 is at 38 kDa.
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