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Immunohistochemical staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells.

Anti-NDEL1 Antibody

HPA017916
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNDEL1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NDEL1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID81565
  • Target name
    NDEL1
  • Target description
    nudE neurodevelopment protein 1 like 1
  • Target synonyms
    endooligopeptidase A; EOPA; MITAP1; mitosin-associated protein 1; mitosin-associated protein MITAP1; NDE1L1; NDE2; nuclear distribution protein nudE-like 1; nudE nuclear distribution E homolog-like 1; nudE nuclear distribution gene E homolog-like 1; NUDEL; protein Nudel
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9GZM8
  • Protein Name
    Nuclear distribution protein nudE-like 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NDEL1 using anti-NDEL1 antibody (A02478-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDEL1 antigen affinity purified polyclonal antibody (Catalog # A02478-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDEL1 at approximately 38 kDa. The expected band size for NDEL1 is at 38 kDa.
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WB analysis of various sample lysates using GTX54566 Nudel antibody. Dilution : 1:1000 Loading : 25microg per lane
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