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Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Anti-NDFIP2 Antibody

HPA009160
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNDFIP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NDFIP2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54602
  • Target name
    NDFIP2
  • Target description
    Nedd4 family interacting protein 2
  • Target synonyms
    N4WBP5A, NEDD4 family-interacting protein 2, MAPK-activating protein PM04 PM05 PM06 PM07, NEDD4 WW domain-binding protein 5A, NF-kappa-B-activating protein 413, putative MAPK-activating protein PM04/PM05/PM06/PM07, putative NF-kappa-B-activating protein 413
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NV92
  • Protein Name
    NEDD4 family-interacting protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of NDFIP2 using anti-NDFIP2 antibody (A08384-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SK-OV-3 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDFIP2 antigen affinity purified polyclonal antibody (Catalog # A08384-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDFIP2 at approximately 39 kDa. The expected band size for NDFIP2 is at 39 kDa.
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