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Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.

Anti-NDOR1 Antibody

HPA024504
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNDOR1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NDOR1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27158
  • Target name
    NDOR1
  • Target description
    NADPH dependent diflavin oxidoreductase 1
  • Target synonyms
    CIAE1, NR1, bA350O14.9, NADPH-dependent diflavin oxidoreductase 1, NADPH-dependent FMN and FAD-containing oxidoreductase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UHB4
  • Protein Name
    NADPH-dependent diflavin oxidoreductase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of NR1/NDOR1 using anti-NR1/NDOR1 antibody (A09352-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR1/NDOR1 antigen affinity purified polyclonal antibody (Catalog # A09352-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR1/NDOR1 at approximately 72 kDa. The expected band size for NR1/NDOR1 is at 67 kDa.
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