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Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Anti-NDUFA13 Antibody

HPA041213
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNDUFA13
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NDUFA13
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51079
  • Target name
    NDUFA13
  • Target description
    NADH:ubiquinone oxidoreductase subunit A13
  • Target synonyms
    B16.6, CDA016, CGI-39, GRIM-19, GRIM19, MC1DN28, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13, CI-B16.6, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13, NADH-ubiquinone oxidoreductase B16.6 subunit, cell death regulatory protein GRIM-19, cell death-regulatory protein GRIM19, complex I B16.6 subunit, complex I-B16.6, gene associated with retinoic and IFN-induced mortality 19 protein, gene associated with retinoic and interferon-induced mortality 19 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P0J0
  • Protein Name
    NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of GRIM19/NDUFA13 using anti-GRIM19/NDUFA13 antibody (A05981-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIM19/NDUFA13 antigen affinity purified polyclonal antibody (Catalog # A05981-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRIM19/NDUFA13 at approximately 16,17 kDa. The expected band size for GRIM19/NDUFA13 is at 16-18 kDa.
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