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Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts.

Anti-NDUFB2 Antibody

HPA051377
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNDUFB2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NDUFB2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4708
  • Target name
    NDUFB2
  • Target description
    NADH:ubiquinone oxidoreductase subunit B2
  • Target synonyms
    AGGG, CI-AGGG, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2, 8kDa, NADH-ubiquinone oxidoreductase AGGG subunit, complex I AGGG subunit, complex I-AGGG
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95178
  • Protein Name
    NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of NDUFB2 using anti-NDUFB2 antibody (A13719-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFB2 antigen affinity purified polyclonal antibody (Catalog # A13719-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFB2 at approximately 15KD. The expected band size for NDUFB2 is at 15KD.
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