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Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.

Anti-NDUFB7 Antibody

HPA002817
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNDUFB7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NDUFB7
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4713
  • Target name
    NDUFB7
  • Target description
    NADH:ubiquinone oxidoreductase subunit B7
  • Target synonyms
    B18; cell adhesion protein SQM1; CI-B18; complex I B18 subunit; complex I-B18; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7; NADH-ubiquinone oxidoreductase B18 subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17568
  • Protein Name
    NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NDUFB7 using anti-NDUFB7 antibody (A10231-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFB7 antigen affinity purified polyclonal antibody (Catalog # A10231-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFB7 at approximately 19 kDa. The expected band size for NDUFB7 is at 16,22 kDa.
Product group Antibodies
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Anti-NDUFB7 Antibody Picoband(r)A10231-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
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WB analysis of HeLa (A), PC12 (B), NS-1 (C) whole cell lysates using GTX56063 NDUFB7 antibody.
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Anti-NDUFB7 Antibody
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Protein IDP17568
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TargetNDUFB7
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