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Immunohistochemical staining of human heart muscle shows strong granular positivity in myocytes.
Immunohistochemical staining of human heart muscle shows strong granular positivity in myocytes.
Immunohistochemical staining of human heart muscle shows strong granular positivity in myocytes.

Anti-NDUFB8 Antibody

HPA003886
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNDUFB8
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NDUFB8
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4714
  • Target name
    NDUFB8
  • Target description
    NADH:ubiquinone oxidoreductase subunit B8
  • Target synonyms
    ASHI; CI-ASHI; complex I ASHI subunit; complex I-ASHI; MC1DN32; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial; NADH:ubiquinone oxidoreductase ASHI subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95169
  • Protein Name
    NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NDUFB8 using anti-NDUFB8 antibody (A07936-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human U937 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: human HL-60 whole cell lysates, Lane 9: rat liver tissue lysates, Lane 10: rat heart tissue lysates, Lane 11: mouse liver tissue lysates, Lane 12: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFB8 antigen affinity purified polyclonal antibody (Catalog # A07936-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFB8 at approximately 19-22 kDa. The expected band size for NDUFB8 is at 22 kDa.
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