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Immunohistochemical staining of human stomach shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows strong granular cytoplasmic positivity in glandular cells.

Anti-NDUFS6 Antibody

HPA064221
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNDUFS6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NDUFS6 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4726
  • Target name
    NDUFS6
  • Target description
    NADH:ubiquinone oxidoreductase subunit S6
  • Target synonyms
    CI-13kA, CI-13kD-A, CI13KDA, MC1DN9, NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial, NADH dehydrogenase (ubiquinone) Fe-S protein 6, 13kDa (NADH-coenzyme Q reductase), NADH-ubiquinone oxidoreductase 13 kDa-A subunit, NADH:ubiquinone oxidoreductase NDUFS6 subunit, complex I 13kDa subunit A, complex I, mitochondrial respiratory chain, 13-kD subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75380
  • Protein Name
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of NDUFS6 using anti-NDUFS6 antibody (A09082-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HUH-7 whole cell lysates, Lane 3: human HCCT tissue lysates, Lane 4: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFS6 antigen affinity purified polyclonal antibody (Catalog # A09082-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFS6 at approximately 14 kDa. The expected band size for NDUFS6 is at 14 kDa.
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