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Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.

Anti-NEBL Antibody

HPA013994
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNEBL
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NEBL Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10529
  • Target name
    NEBL
  • Target description
    nebulette
  • Target synonyms
    C10orf113, LASP2, LNEBL, bA165O3.1, nebulette, LIM and SH3 protein 2, LIM-nebulette, actin-binding Z-disk protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO76041
  • Protein Name
    Nebulette
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
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Figure 1. Western blot analysis of NEBL using anti-NEBL antibody (A08817-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEBL antigen affinity purified polyclonal antibody (Catalog # A08817-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEBL at approximately 126 kDa. The expected band size for NEBL is at 116,126 kDa.
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