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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Anti-NECTIN3 Antibody

HPA011038
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetNECTIN3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NECTIN3
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25945
  • Target name
    NECTIN3
  • Target description
    nectin cell adhesion molecule 3
  • Target synonyms
    CD113; CDW113; nectin 3; nectin-3; poliovirus receptor-related 3; poliovirus receptor-related protein 3; PPR3; PRR3; PVRL3; PVRR3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NQS3
  • Protein Name
    Nectin-3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Nectin 3/NECTIN3 using anti-Nectin 3/NECTIN3 antibody (A09633-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates. Lane 2: human HEK293 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nectin 3/NECTIN3 antigen affinity purified polyclonal antibody (Catalog # A09633-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nectin 3/NECTIN3 at approximately 80KD. The expected band size for Nectin 3/NECTIN3 is at 61KD.
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